Each individual submitting samples to be sequenced should create their own user profile. We will use this information to notify clients when their samples are complete and for billing purposes. Be sure that all starred ( * ) fields are completed.

Login to the automated sample submission/retrieval site using your e-mail address and password used in creating your user profile. Double check your profile to be sure that all information is correct. The e-mail address entered will be the one used to update you on sample progress.

Enter the appropriate data in each field for every sample. It is important that all data for each reaction is completed. The only field that can be left blank is the 'comment' field. The product length field applies to both plasmid and PCR templates. Specify any useful information for reactions in 'comment' column; i.e. G-C rich template, high primer Tm.

After you have entered all the data for your samples, press the 'submit' button.
A confirmation page is generated summarizing all of your sample information. Please print out a copy of this page to submit with your DNA samples. Also make a copy for your records. Each template/primer to be sequenced is assigned a sample ID number. The sample ID number becomes the name of the sample file which contains the sequencing results for your reaction. (Ex. Template name = pGEM, primer name = m13rev, assigned ID number = 321, and results for the sequencing of pGEM with m13rev will be in file 321.ABI)

DEADLINES: The cutoff time for electronic submission of samples is 10:30AM. Samples entered after 10:30AM will be processed on the next business day. The deadline for DNA samples to arrive in the Core Facility for same day processing is 11:00AM. DNA samples arriving after 11:00AM (even if the 10:30AM electronic submission cutoff time is met) will not be processed until the next business day.

DNA samples and their primers should be brought to the Genomics Core in Kaul 412 and left in the appropriate plastic box in the refrigerator. Boxes are labeled with the days of the week (Monday, Tuesday, etc.) Samples should be left in the box corresponding to the day of the week on which they are submitted. All samples should be labeled clearly with the investigators name and sample ID. Please follow the instructions below for the concentrations and volumes of both sample and primer to submit. Please leave a copy of your sequencing confirmation sheet in the file tray located above the PCR machines. Your lab also should keep a logbook with detailed description of the sample, its number, date of submission, and investigator's name.

Please note: If you are submitting a template to be sequenced with several primers or vice-versa, please submit only one tube for all of the reactions (not multiple tubes containing the same template or primer).

You will receive an e-mail when your results are ready to download.
If you do not have an electropherogram viewing program (Chromas or Edit View) installed on your computer click here for further instructions.

When you login to the automated sample submission/retrieval site, the page displayed will be the completed samples page. At the right end of each sample line there is the symbol 'ABI file'. Clicking on the 'ABI file' opens a window which asks whether you want to open the file or save it to disk. Save the file to disk. Once you have saved all of your files, logout of the automated sample submission/retrieval site and open your sequence files using your electropherogram viewing program.